Related Experiment Video
Updated: Sep 22, 2025
13:08
Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
Published on: September 9, 2011
27.8K
Titanium alkalinization improves response of osteoblasts to zoledronic acid
Rodrigo Paes Vila Real1, Taisa Nogueira Pansani2, Lais Medeiros Cardoso3
1Department of Dentistry, Ribeirão Preto University (UNAERP), Ribeirão Preto, SP 14096-900, Brazil.
Biointerphases
|May 26, 2022
Summary
NaOH modification of titanium surfaces enhances osteoblast metabolism and viability, even when treated with zoledronic acid (ZA). This surface treatment shows potential for improving osseointegration in patients undergoing ZA therapy.
Area of Science:
- Biomaterials Science
- Orthopedic Research
- Cell Biology
Background:
- Titanium is a common implant material, but its surface properties can influence osseointegration.
- Zoledronic acid (ZA) is used to treat bone diseases but can negatively affect bone cell metabolism.
- Optimizing titanium surface characteristics is crucial for enhancing implant success, especially in patients on ZA therapy.
Purpose of the Study:
- To investigate the effect of NaOH surface modification on titanium disks.
- To evaluate the impact of this modification on osteoblast metabolism and differentiation.
- To determine if NaOH-treated titanium improves osteoblast response in the presence of zoledronic acid (ZA).
Main Methods:
- Titanium disks were treated with NaOH and characterized using SEM, confocal microscopy, and XPS.
- Human osteoblasts were cultured on machined and NaOH-treated disks.
- Cells were treated with zoledronic acid (ZA) for 7 days.
- Cell viability, collagen synthesis, protein production, alkaline phosphatase activity, and mineral nodule deposition were assessed.
Main Results:
- NaOH treatment increased titanium surface roughness and altered surface chemistry.
- Modified surfaces showed significantly higher osteoblast viability, collagen synthesis, protein production, and alkaline phosphatase activity compared to machined surfaces.
- While ZA reduced cellular metabolism on both surfaces, the NaOH-modified surfaces maintained higher metabolic activity and differentiation markers.
- Mineral nodule deposition was an exception, showing no significant difference between surfaces in the presence of ZA.
Conclusions:
- NaOH surface modification of titanium enhances osteoblast adhesion, metabolism, and differentiation.
- This surface treatment mitigates some negative effects of zoledronic acid (ZA) on osteoblasts.
- NaOH-modified titanium holds promise for improving osseointegration, particularly for patients receiving ZA treatment.
More Related Videos
Related Concept Videos
01:25Role of Vitamins in Maintaining Bone Health
3.7K
The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
3.7K
01:31Osteoclasts in Bone Remodeling
3.2K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.2K
01:40Bone Remodeling
38.6K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.6K
01:18The Bone Matrix
4.6K
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in...
4.6K