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Related Experiment Video

Updated: Sep 21, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
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SARS-CoV-2 detection using a nanobody-functionalized voltammetric device.

Quentin Pagneux1, Alain Roussel2, Hiba Saada1

  • 1Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, Lille, France.

Communications Medicine
|May 27, 2022
PubMed
Summary
This summary is machine-generated.

A new nanobody-based electrochemical sensor rapidly detects SARS-CoV-2 in saliva and swabs within 10 minutes. This accurate COVID-19 test differentiates viral strains and variants, offering a powerful point-of-care diagnostic tool.

Keywords:
Diagnostic markersNanobiotechnology

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Area of Science:

  • Electrochemistry
  • Nanotechnology
  • Infectious Disease Diagnostics

Background:

  • Accurate point-of-care testing is crucial for differentiating SARS-CoV-2 from other viruses.
  • Electrochemical platforms offer potential for virus detection via spike protein but face sensitivity challenges.

Purpose of the Study:

  • To develop a rapid, sensitive, and specific electrochemical platform for detecting whole SARS-CoV-2 viral particles.
  • To enable differentiation of SARS-CoV-2 from other respiratory viruses at the point-of-care.

Main Methods:

  • Functionalization of a gold electrode surface with Llama nanobodies specific to the SARS-CoV-2 spike protein receptor binding domain (RBD).
  • Detection of whole SARS-CoV-2 viral particles in unprocessed saliva and nasopharyngeal swab samples.
  • Voltammetric signal analysis for rapid viral detection.

Main Results:

  • The nanobody-functionalized sensor detected SARS-CoV-2 in 10 minutes using 200 µL of unprocessed samples.
  • High specificity was observed for SARS-CoV-2 in human saliva and nasopharyngeal swabs.
  • The sensor achieved 90% positive and 90% negative agreement with RT-qPCR on 80 clinical samples, distinguishing various human coronaviruses and respiratory viruses.

Conclusions:

  • The developed electrochemical diagnostic platform is a powerful tool for detecting SARS-CoV-2 infection status.
  • The platform demonstrated validation for RBD mutants and successful testing on the Delta variant.
  • The diagnostic concept is extendable to other viruses and can overcome sensing challenges related to viral mutations.