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Updated: Sep 21, 2025

Isolation, Processing and Analysis of Murine Gingival Cells

Isolation, Processing and Analysis of Murine Gingival Cells

Published on: July 2, 2013

Dissecting B/Plasma Cells in Periodontitis at Single-Cell/Bulk Resolution.

L Liu^1,2, Y Chen¹, L Wang^1,2

¹State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.

Journal of Dental Research

|May 27, 2022

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This study reveals how immune cells interact in periodontitis, identifying a B/plasma cell signature for potential molecular diagnosis of this gum disease.

Area of Science:

Immunology
Genomics
Oral Biology

Background:

Periodontitis understanding has advanced from gross to molecular levels.
Transcriptomic studies offer insights but lack detailed immune cell communication analysis.
Current research needs finer dissection of immune cell subtypes and their biological processes in periodontitis.

Purpose of the Study:

To classify immune cell types and analyze cell communication in periodontitis at a single-cell level.
To investigate B/plasma cell infiltration and identify distinct cell fates.
To develop and validate molecular diagnostic models for periodontitis using transcriptomic data.

Main Methods:

Single-cell transcriptome profiling of 15 immune cell types in periodontitis.
Cell communication analysis, pseudotime analysis, and gene set enrichment analysis.

Keywords:

bioinformatics immunity logistic models oral diagnosis periodontal disease(s)/periodontitis single-cell sequencing

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Last Updated: Sep 21, 2025

Isolation, Processing and Analysis of Murine Gingival Cells

Isolation, Processing and Analysis of Murine Gingival Cells

Published on: July 2, 2013

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Published on: February 16, 2016

Machine learning, multivariable logistic regression, and quantitative real-time polymerase chain reaction validation.

Main Results:

Identified macrophage migration inhibitory factor communication from plasma cells to other immune cells.
Discovered two distinct cell fates for B/plasma cells during infiltration.
Developed a CF1 gene signature demonstrating good discrimination for periodontitis molecular diagnosis.

Conclusions:

Transcriptomic landscape analysis clarifies B/plasma cell infiltration in periodontitis.
A novel gene signature shows promise for assisting in the molecular diagnosis of periodontitis.
Further validation of gene candidates in gingival tissues and fluids supports diagnostic potential.