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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Sep 21, 2025

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Laser particle activated cell sorting in microfluidics.

Paul H Dannenberg^1,2,3, Jisoo Kang¹, Nicola Martino^1,2

¹Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA.

|May 27, 2022

Summary

This summary is machine-generated.

Researchers developed laser particle activated cell sorting (LACS) using microfluidics and spectroscopy. This technique enables high-speed, spectrally distinct cellular barcoding and sorting for advanced single-cell analysis.

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Last Updated: Sep 21, 2025

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

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Published on: January 20, 2023

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Area of Science:

Biotechnology
Spectroscopy
Microfluidics

Background:

Laser particles offer bright, spectrally narrowband emission suitable for cellular barcoding.
Distinguishing cells based on optical barcodes is crucial for single-cell analysis.

Purpose of the Study:

To demonstrate a microfluidic platform for reading laser particle emission and routing cells based on optical barcodes.
To introduce laser particle activated cell sorting (LACS) for high-speed, spectrally distinct cellular analysis.

Main Methods:

Integration of a microfluidic platform with a high-speed spectrometer.
Utilizing sub-nanometer spectral emission of laser particles for near-infrared cellular barcoding.
Employing a real-time field-programmable gate array and electric field switch for kilohertz-rate sorting.

Main Results:

Demonstrated the ability to distinguish individual cells labeled with hundreds of different laser colors.
Achieved kilohertz-rate sorting of cells based on their spectral barcodes.
Successfully performed various sorting functions, including isolation of barcoded cells and cells with specific laser colors.

Conclusions:

Laser particle activated cell sorting (LACS) is a novel technique for single-cell analysis.
This technology enhances the capabilities of existing single-cell analysis tools by enabling high-speed, spectrally encoded cell sorting.