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Related Concept Videos

Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

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Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
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Immunoprecipitation01:20

Immunoprecipitation

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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
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T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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Related Experiment Video

Updated: Sep 21, 2025

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
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Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

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Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics.

James Peter Stewart1, Jana Gazdova1, Shambhavi Srivastava1

  • 1Patrick G Johnston Centre for Cancer Research, Queen's University Belfast, Belfast, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 27, 2022
PubMed
Summary

The EuroClonality-NGS DNA Capture assay offers a comprehensive solution for detecting genetic biomarkers in hemato-oncology. This integrated approach improves diagnostic accuracy and efficiency in clinical molecular diagnostics.

Keywords:
BIOMED-2Copy number alterationEuroClonalityImmunoglobulinLymphomaNext generation sequencingT cell receptorTranslocation

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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

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Related Experiment Videos

Last Updated: Sep 21, 2025

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Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

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Area of Science:

  • Genomic medicine
  • Molecular diagnostics
  • Hemato-oncology

Background:

  • Targeted next-generation sequencing (NGS) is crucial for identifying genetic biomarkers in hemato-oncology.
  • Clinical molecular diagnostic laboratories increasingly adopt NGS strategies.

Purpose of the Study:

  • To describe the EuroClonality-NGS DNA Capture (EuroClonality-NDC) assay.
  • To highlight its capability for simultaneous detection of multiple genetic alterations.
  • To present an integrated bioinformatics pipeline for reporting.

Main Methods:

  • Development and validation of the EuroClonality-NDC assay.
  • Simultaneous detection of B and T cell clonal rearrangements, translocations, copy number alterations, and sequence variants.
  • Utilizing a validated bioinformatics pipeline for integrated reporting.

Main Results:

  • The EuroClonality-NDC assay enables simultaneous detection of key genetic alterations.
  • The integrated bioinformatics pipeline produces comprehensive reports.
  • The combined approach minimizes human error and reduces costs.

Conclusions:

  • The EuroClonality-NDC assay provides an efficient and cost-effective molecular testing strategy.
  • This integrated assay and pipeline are expected to improve diagnostic outcomes in hemato-oncology.
  • It represents an advancement in clinical molecular diagnostics for blood cancers.