AIRR Community Guide to Planning and Performing AIRR-Seq Experiments.

Area of Science:

• Immunology
• Genomics
• Bioinformatics

Background:

• Adaptive immune receptor sequencing (AIRR-seq) has rapidly advanced, generating vast amounts of data on immune system dynamics.
• Numerous protocols, experimental methods, and computational tools have emerged, creating a need for standardized guidance.

Purpose of the Study:

• To provide a comprehensive overview of planning and executing successful AIRR-seq experiments.
• To outline strategies for controlling and evaluating experimental outcomes in immune receptor repertoire analysis.
• To guide researchers in conducting, analyzing, and sharing AIRR-seq projects effectively.

Main Methods:

• Discussion of key considerations for designing AIRR-seq experiments.
• Presentation of basic strategies for quality control and outcome evaluation.
• Reference to detailed, step-by-step instructions provided by AIRR Community members.

Main Results:

• The chapter synthesizes current knowledge and best practices in AIRR-seq.
• It highlights the importance of careful planning and rigorous evaluation for reliable results.
• It serves as a foundational resource for researchers entering the field.

Conclusions:

• Successful AIRR-seq requires meticulous experimental design and validation.
• Standardized approaches are crucial for advancing the analysis of adaptive immune repertoires.
• This work facilitates reproducible and impactful research in immunology and bioinformatics.