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Visualizing G protein-coupled receptor homomers using photoactivatable dye localization microscopy

Uchechukwu Agwuegbo1, Kim Carol Jonas1

  • 1Department of Women and Children's Health, School of Life Course Sciences, King's College London, Guy's Campus, London, United Kingdom.

Methods in Cell Biology
|May 27, 2022
PubMed

Keywords:
DimerizationG protein-coupled receptorOligomerizationPD-PALMSingle-molecule imaging

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G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
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