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FIBS-enabled Noninvasive Metabolic Profiling

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Intein-Mediated Protein Engineering for Cell-Based Biosensors.

Chungwon Kang¹, Keshab Lal Shrestha¹, San Kwon¹

¹Department of Biomedical Engineering, Dongguk University, Seoul 04260, Korea.

|May 28, 2022

Summary

Cell-based sensors enable live-cell target screening and signal visualization. Intein-mediated protein engineering enhances these biosensors for improved drug discovery and cell-signaling research.

Keywords:

bio-recognition element biosensors cell-based sensors conditional protein splicing reporter element split-intein

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Area of Science:

Biotechnology
Molecular Biology
Cell Biology

Background:

Cell-based sensors offer a versatile platform for screening biological targets and monitoring cellular interactions in real-time.
They are crucial in drug discovery and cell-signaling research, enabling function-based screening in native cellular environments.

Purpose of the Study:

To review current cell-based sensor technologies and their limitations.
To highlight the application of intein-mediated protein engineering in advancing cell-based sensor fabrication.

Main Methods:

Discussion of cell-based sensor characteristics and design strategies, differentiating them from in vitro biosensors.
Description of intein-mediated protein engineering for fabricating advanced cell-based sensors.
Explanation of intein-mediated reaction mechanisms and their role in sensor development.

Main Results:

Intein-mediated protein engineering offers novel approaches to overcome limitations in current cell-based sensor technologies.
This technology facilitates the development of improved methods for sensor cell fabrication.
Real-time in vivo visualization of cellular signals with high spatiotemporal resolution is achievable.

Conclusions:

Intein-mediated protein engineering is a powerful tool for enhancing cell-based sensor capabilities.
These advancements are critical for accelerating drug discovery and deepening our understanding of cell signaling pathways.