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In Situ Electroporation on PERFECT Filter for High-Efficiency and High-Viability Tumor Cell Labeling.

Tingting Hun1, Yi Zhang1, Qingmei Xu1

  • 1School of Integrated Circuits, Peking University, Beijing 100871, China.

Micromachines
|May 28, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a novel in situ electroporation system for efficient enrichment and labeling of rare circulating tumor cells (CTCs). The method enhances CTC tracking for tumor metastasis research with high efficiency and cell viability.

Keywords:
circulating tumor cellhigh viabilityin situ electroporation

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Cancer Research

Background:

  • Circulating tumor cells (CTCs) are crucial for understanding tumor metastasis.
  • Efficient enrichment and labeling of rare CTCs from blood are challenging but necessary for mechanistic studies.
  • Existing methods often face limitations in speed, efficiency, and cell viability.

Purpose of the Study:

  • To develop and validate a novel in situ electroporation system integrated with a micropore-arrayed filter (PERFECT filter) for simultaneous CTC enrichment and labeling.
  • To assess the efficiency, specificity, and cell viability of the proposed system for CTC labeling.
  • To demonstrate the potential of the system for rapid CTC tracking in cancer research.

Main Methods:

  • Development of an in situ electroporation technique on a PERFECT filter for rare cell enrichment.
  • Optimization of electroporation parameters (electric field strength, pulse number) for enhanced molecular delivery.
  • Verification of labeling efficiency and cell viability using various biomolecules and live/dead staining.
  • Assessment of labeling specificity using fluorescent dye-conjugated antibodies.

Main Results:

  • The PERFECT filter enabled efficient enrichment of rare tumor cells with reduced damage and uniform size distribution.
  • High labeling efficiency (74.08 ± 2.94%) and cell viability (81.15 ± 3.04%) were achieved through optimized electroporation.
  • High specificity (99.03 ± 1.67%) for tumor cell probing was demonstrated.
  • The entire process, including cell separation and electroporation, was completed in under 10 minutes.

Conclusions:

  • The proposed in situ electroporation on the PERFECT filter system offers a rapid and efficient method for simultaneous enrichment and labeling of CTCs.
  • This technology significantly enhances molecular delivery and preserves cell viability, making labeled CTCs suitable for further analysis and culture.
  • The system holds great potential for advancing CTC tracking and understanding tumor metastasis mechanisms.