Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Melatonin-Induced Modulation of Cholesterol-Enriched Model Neuronal Membranes.

ACS chemical neuroscience·2026
Same author

Ion transport through reconfigurable nanoparticle-surfactant stabilized droplet interface bilayers.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Real-Time In Vivo Detection of Nanocarrier Number and Velocity in the Cerebrovasculature Using Hot Band Absorption.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

M1 Macrophage-Engineered Vesicles Have Anti-Cancer Activity in Ovarian Cancer.

Cancer nanotechnology·2026
Same author

Electromechanically induced membrane restructuring enables learning and memory.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Improving Proteostasis of Trafficking-Deficient GABA<sub>A</sub> Receptor Variants by Activating IRE1.

ACS chemical neuroscience·2025

Related Experiment Video

Updated: Sep 21, 2025

Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy
08:01

Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy

Published on: May 12, 2020

8.2K

Gold Ion Beam Milled Gold Zero-Mode Waveguides.

Troy C Messina1, Bernadeta R Srijanto2, Charles Patrick Collier2

  • 1Department of Physics, Berea College, 101 Chestnut Street, Berea, KY 40404, USA.

Nanomaterials (Basel, Switzerland)
|May 28, 2022
PubMed
Summary
This summary is machine-generated.

Focused ion beam milling offers a new method for creating zero-mode waveguides (ZMWs). Gold ZMWs produced this way show enhanced fluorescence, matching traditional fabrication techniques for single-molecule microscopy.

Keywords:
nanostructuressingle moleculesingle molecule spectroscopysub-wavelength apertureszero-mode waveguides

More Related Videos

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
12:14

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

Published on: August 12, 2013

21.9K
Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments
06:40

Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments

Published on: January 28, 2021

4.4K

Related Experiment Videos

Last Updated: Sep 21, 2025

Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy
08:01

Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy

Published on: May 12, 2020

8.2K
The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
12:14

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

Published on: August 12, 2013

21.9K
Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments
06:40

Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments

Published on: January 28, 2021

4.4K

Area of Science:

  • Optics and Photonics
  • Materials Science
  • Biophysics

Background:

  • Zero-mode waveguides (ZMWs) enhance fluorescence and enable single-molecule studies at physiological concentrations.
  • Traditional ZMW fabrication relies on photolithography or electron beam lithography.
  • Plasmon enhancement is a key feature of ZMWs for sensitive detection.

Purpose of the Study:

  • To introduce a novel fabrication method for zero-mode waveguides (ZMWs) using focused ion beam (FIB) milling.
  • To investigate the optical properties of ZMWs fabricated with gold ions.
  • To compare the performance of FIB-milled ZMWs with those made by conventional lithography.

Main Methods:

  • Focused ion beam (FIB) milling with gold ions was employed for ZMW fabrication.
  • Fabrication of ZMWs with 200 nm apertures.
  • Characterization of plasmon-enhanced fluorescence from the fabricated ZMWs.

Main Results:

  • Successful fabrication of gold ZMWs using FIB milling.
  • Demonstration of plasmon-enhanced fluorescence in ion-milled gold ZMWs.
  • Comparable fluorescence enhancement to ZMWs produced by electron beam lithography.

Conclusions:

  • Focused ion beam milling presents a viable alternative for ZMW fabrication.
  • Ion-milled gold ZMWs offer similar optical performance to traditionally fabricated devices.
  • This method expands fabrication options for single-molecule fluorescence microscopy applications.