Ascorbic Acid Preconditioning Effect on Broccoli Seedling Growth and Photosynthesis under Drought Stress

Drought is an abiotic stress that decreases crop photosynthesis, growth, and yield. Ascorbic acid has been used as a seed preconditioning agent to help mitigate drought in some species, but not yet in broccoli (Brassica oleracea var. italica). The objective was to investigate the effect of ascorbic acid on growth, photosynthesis, and related parameters in watered and drought-stressed broccoli seedlings. A 2 × 4 factorial experiment was designed where stress (watered or drought) was the first factor and ascorbic acid preconditioning (untreated, 0 ppm, 1 ppm, or 10 ppm) was the second factor. Positioning within the greenhouse was included as a blocking factor and the experiment was replicated three times. All seedlings were watered for 8 weeks and then half had water withheld for 7 days to impose drought while the other half continued to be watered. Ascorbic acid preconditioning increased shoot dry mass, root dry mass, water use efficiency, and photosynthesis in all seedlings while also increasing chlorophyll, relative water content, and leaf area in droughted seedlings. Ascorbic acid preconditioning also decreased membrane injury in droughted seedlings to the point that membrane injury was not significantly different than the watered control. There was strong evidence to support ascorbic acid as a successful seed preconditioning agent in watered and droughted broccoli.