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Generation of Sigmar1 conditional knockout mouse using CRISPR-Cas9 gene targeting
Liang Huang1,2, Haiyan Xiao2,3, Xiaoling Xie1
1Department of Neuroscience and Regenerative Medicine, Augusta University Medical College of Georgia, Augusta, Georgia, USA.
Genesis (New York, N.Y. : 2000)
|May 28, 2022
Summary
Researchers developed new conditional knockout mice for the Sigma 1 receptor (SIGMAR1). These mice allow for precise investigation into SIGMAR1
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- The Sigma 1 receptor (SIGMAR1) is crucial for cell survival and implicated in neurodegeneration.
- Its precise function across different tissues and cell types is not fully understood.
Purpose of the Study:
- To generate a conditional knockout mouse model for SIGMAR1.
- To enable tissue- and cell-type-specific studies of SIGMAR1 function.
Main Methods:
- CRISPR-Cas9 technology was used to insert loxP sites into the Sigmar1 gene.
- Conditional knockout mice (Sigmar1loxP) were generated.
- These mice were crossed with Six3-Cre and CMV-Cre mice for retina-specific and global knockouts, respectively.
Main Results:
- LoxP insertion did not impact Sigmar1 expression or cause visual defects in early adult mice.
- Successful deletion of Sigmar1 exons 1-4 was confirmed.
- Retina-specific and global loss of SIGMAR1 expression was achieved in respective crosses.
Conclusions:
- The generated Sigmar1 conditional knockout mice are a valuable research tool.
- This model facilitates detailed investigation into the specific roles of SIGMAR1 in various biological contexts.
