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Correlation between Intestinal Microflora in Irritable Bowel Syndrome and Severity
Min Ji1, Hua Huang1, Xianming Lan1
1The Second People's Hospital of Longgang District, Shenzhen, Guangdong 518112, China.
Disease Markers
|May 31, 2022
Summary
Gut microbiota alterations, including lower levels of beneficial bacteria and higher levels of harmful bacteria, are linked to irritable bowel syndrome (IBS) severity. These changes correlate with elevated serum D-lactate and diamine oxidase (DAO) levels.
Area of Science:
- Gastroenterology and Microbiology
- Functional Gastrointestinal Disorders
- Human Gut Microbiome Research
Background:
- Irritable bowel syndrome (IBS) is a prevalent chronic functional gastrointestinal disorder characterized by alterations in intestinal microecology.
- Understanding the gut microbiota's role in IBS pathogenesis and severity is crucial for developing targeted therapies.
Purpose of the Study:
- To investigate the association between gut microbiota composition and the severity of Irritable Bowel Syndrome (IBS).
- To explore the relationship between specific gut bacteria, serum D-lactate, and diamine oxidase (DAO) levels in IBS patients.
- To determine how these factors correlate with the clinical presentation and progression of IBS.
Main Methods:
- An observational study involving 60 IBS patients and 20 healthy controls.
- Analysis of fecal samples to quantify key intestinal flora: Bifidobacterium, Lactobacillus, Enterococcus, and Enterobacter.
- Measurement of serum D-lactate and diamine oxidase (DAO) levels to assess intestinal barrier function and inflammation.
Main Results:
- IBS patients exhibited lower levels of Lactobacillus and Bifidobacterium, and higher levels of Enterococcus and Enterobacter compared to controls.
- Moderate to severe IBS cases showed significantly lower beneficial bacteria and higher opportunistic bacteria, along with elevated D-lactate and DAO levels.
- A strong negative correlation was observed between beneficial bacteria and IBS severity, while opportunistic bacteria, D-lactate, and DAO showed a positive correlation.
Conclusions:
- Significant alterations in gut microbiota composition are evident in IBS patients.
- Gut microbiota dysbiosis is closely correlated with IBS disease severity.
- Elevated D-lactate and DAO levels in IBS patients are linked to specific gut microbial imbalances and disease progression.
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