Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetics of Speciation02:16

Genetics of Speciation

19.7K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
19.7K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

443
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
443
Formation of Species01:31

Formation of Species

42.8K
Speciation describes the formation of one or more new species from one or sometimes multiple original species. The resulting species are discrete from the parent species, and barriers to reproduction will typically exist. There are two primary mechanisms, speciation with and without geographic isolation—allopatric and sympatric speciation, respectively.
42.8K
CRISPR01:59

CRISPR

53.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.1K
Hybrid Zones02:29

Hybrid Zones

20.5K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
20.5K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

6.2K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Computer-Aided Sperm Analysis Protocol for Evaluating Sperm Motility in Japanese Medaka.

Development, growth & differentiation·2026
Same author

A genetic model of congenital intestinal atresia implicates Mypt1 in epithelial organisation.

Disease models & mechanisms·2026
Same author

The evolutionary genomics of meiotic drive.

Molecular biology and evolution·2026
Same author

Shared and Unique Patterns of Genomic Differentiation and Introgression Between Japanese Stickleback Species Across Three Sympatric Sites.

Molecular ecology·2026
Same author

Inferring the strength of directional selection on armor plates in Lake Washington stickleback while accounting for migration and drift.

Evolution; international journal of organic evolution·2025
Same author

Genetic basis of latitudinal variation in vertebral number in the Oryzias latipes species complex.

Zoological letters·2025

Related Experiment Video

Updated: Sep 21, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.4K

Speciation and adaptation research meets genome editing.

Satoshi Ansai1, Jun Kitano2

  • 1Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, Miyagi 980-8577, Japan.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|May 31, 2022
PubMed
Summary

Genome editing tools like CRISPR enable functional analysis of genetic variations in natural populations. These techniques are crucial for understanding the genetic basis of speciation and adaptation.

Keywords:
CRISPR-Caschromosomal rearrangementgenome editingknock-inknock-outsite-specific recombination

More Related Videos

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
08:51

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Published on: May 13, 2016

14.1K
Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs
07:42

Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs

Published on: June 20, 2016

8.4K

Related Experiment Videos

Last Updated: Sep 21, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.4K
Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
08:51

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Published on: May 13, 2016

14.1K
Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs
07:42

Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs

Published on: June 20, 2016

8.4K

Area of Science:

  • Evolutionary Biology
  • Genetics
  • Genomics

Background:

  • Understanding the genetic underpinnings of reproductive isolation and adaptive traits in natural populations is a key challenge in evolutionary biology.
  • Genome editing technologies, including CRISPR-Cas systems and site-specific recombinases, offer powerful tools for targeted genomic modification.

Purpose of the Study:

  • To review the technical properties and applications of genome editing techniques for functional analysis of genetic variations.
  • To explore the utility of these technologies in advancing research on speciation and adaptation.

Main Methods:

  • Classification of genome editing applications: targeted gene knock-out, knock-in, precise gene replacement, and chromosomal rearrangement.
  • Review of technical properties and examples of application in functional analysis of natural genetic variations.

Main Results:

  • Genome editing allows for precise modification of targeted genomic regions in non-conventional model organisms.
  • Applications include investigating gene functions, visualizing expression patterns, identifying causative alleles, and analyzing chromosomal structural variations.

Conclusions:

  • Genome editing technologies are instrumental for dissecting the genetic basis of adaptation and speciation.
  • These tools facilitate the functional analysis of naturally occurring genetic variations, advancing evolutionary biology research.