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Updated: Sep 21, 2025

High-Resolution Comparison of Bacterial Conjugation Frequencies
Published on: January 10, 2019
Live-Cell Visualization of DNA Transfer and Pilus Dynamics During Bacterial Conjugation
Kelly Goldlust1, Agathe Couturier1, Laurent Terradot1
1Microbiologie Moléculaire et Biochimie Structurale (MMSB), Université Lyon 1, CNRS, Inserm, UMR5086, Lyon Cedex 07, France.
Abstract:
Bacterial genomes are highly plastic and evolve rapidly by acquiring new genetic information through horizontal gene transfer mechanisms. Capturing DNA transfer by conjugation between bacterial cells in real time is relevant to address bacterial genomes' dynamic architecture comprehensively. Here, we describe a method allowing the direct visualization of bacterial conjugation in live cells, including the fluorescent labeling of the conjugative pilus and the monitoring of plasmid DNA transfer from donor to recipient cells.
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