Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt secretion,...
Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Silicone-Based Polyurethane for Visual Damage Sensing: The Critical Role of Chemical Bonding in Mechanochromic Soft Materials.

Chemistry, an Asian journal·2026
Same author

Impact of the polymer side chain chemistry on interactions of amino-acid-derived polyelectrolytes with cells.

Journal of materials chemistry. B·2026
Same author

Encapsulation of the novel mPGES-1 inhibitor TG554 in acetalated dextran nanoparticles.

RSC advances·2026
Same author

A Dual-Role Amphiphilic Photosensitizer: Enhancing Structural Uniformity and Optical Properties of Langmuir Monolayers.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Multifunctional Elastic Supramolecular Hydrogels with Self-Healing, Adhesive, and Ionic Thermoelectric Property for Flexible Sensing and Energy Harvesting.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

An Innovative Oral Ex Vivo Biofilm Model for Antimicrobial Investigations.

Pathogens (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jun 19, 2026

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

9.6K

Cell-Penetrating, Peptide-Based RAFT Agent for Constructing Penetration Enhancers.

Chao Chen1, Friederike Richter2,3, Carlos Guerrero-Sanchez2,3

  • 1Beijing Advanced Innovation Center for Soft Matter Science and Engineering; College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

ACS Macro Letters
|May 31, 2022
PubMed
Summary

Researchers created novel peptide-polymer conjugates using a cell-penetrating peptide (Transportan 10) and reversible addition-fragmentation chain transfer polymerization. These conjugates effectively penetrate cells, showing promise for drug and gene delivery applications.

More Related Videos

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

6.6K
Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
10:12

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

2.3K

Related Experiment Videos

Last Updated: Jun 19, 2026

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

9.6K
Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

6.6K
Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
10:12

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

2.3K

Area of Science:

  • Polymer Chemistry
  • Bioconjugation
  • Nanotechnology

Background:

  • Peptide-polymer conjugates offer solutions for therapeutic and diagnostic limitations of peptides.
  • Well-defined conjugates are crucial for developing functional drugs and gene delivery systems.

Purpose of the Study:

  • To design and synthesize novel peptide-polymer conjugates using a sequence-defined cell-penetrating peptide.
  • To create amphiphilic block copolymers with cell-penetrating capabilities for enhanced delivery.

Main Methods:

  • Automated peptide synthesis of a Transportan 10 (TP 10)-based chain transfer agent (TP-CTA).
  • Reversible addition-fragmentation chain transfer (RAFT) polymerization to synthesize poly[oligo(ethylene glycol) methyl ether acrylate]-block-poly(n-butyl acrylate) (TP-POEGA-b-PBA) using TP-CTA.
  • Circular dichroism (CD) spectroscopy and transmission electron microscopy (TEM) for characterization.

Main Results:

  • Confirmation of the preserved α-helix structure of TP 10 using CD spectroscopy, crucial for bioactivity.
  • TEM revealed self-assembled nanostructures (rods and vesicles) in aqueous environments.
  • Demonstrated effective cell penetration by the synthesized peptide-conjugated block copolymers.

Conclusions:

  • A novel method for synthesizing peptide-polymer conjugates with cell-penetrating abilities was successfully developed.
  • The synthesized TP-POEGA-b-PBA conjugates show significant potential for applications in drug and gene delivery.
  • Preservation of peptide bioactivity within the polymer conjugate is achievable.