Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AI-guided CRISPR screening reveals therapeutic targets in psoriasis.

Nature communications·2026
Same author

Glioblastoma stem cell growth requires DOT1L-MED23 control of enhancer accessibility.

Cell reports·2026
Same author

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same author

Clarifying Frizzled 2 function in development through genetically validated mouse models.

Disease models & mechanisms·2026
Same author

Integrating Transcription Factors with Electrochemical Pendulum Bioanalysis for Hormone Detection.

Journal of the American Chemical Society·2026
Same author

Automated synthesis of InSb quantum dots with improved batch-to-batch reproducibility via kinetically matched co-reduction.

Nature communications·2026

Related Experiment Video

Updated: Sep 21, 2025

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

12.2K

Nanoparticle Amplification Labeling for High-Performance Magnetic Cell Sorting.

Zongjie Wang1,2, Hansen Wang3, Sichun Lin3,4

  • 1The Edward S. Rogers Sr. Department of Electrical & Computer Engineering, University of Toronto, Toronto M5S 3G4, Canada.

Nano Letters
|May 31, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method to amplify magnetic cell labeling, improving the isolation of rare cells. This technique enhances magnetic cell sorting for applications like cancer diagnosis and immunotherapy.

Keywords:
cancer diagnosiscell sortingcell therapymagnetic nanoparticlesmicrofluidics

More Related Videos

Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

21.3K
Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
09:28

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Published on: November 17, 2018

11.7K

Related Experiment Videos

Last Updated: Sep 21, 2025

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

12.2K
Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

21.3K
Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
09:28

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Published on: November 17, 2018

11.7K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Immunology

Background:

  • Magnetic cell sorting (MACS) is crucial for isolating specific cell types.
  • Efficient MACS requires substantial magnetic nanoparticle labeling, challenging for weakly expressed biomarkers.
  • Current methods face limitations in sensitivity for rare cell populations.

Purpose of the Study:

  • To develop a novel approach for amplifying magnetic cell labeling.
  • To enhance the efficiency and sensitivity of magnetic cell sorting.
  • To enable the isolation of rare cell subpopulations for diagnostic and therapeutic applications.

Main Methods:

  • Sequential antibody-nanoparticle conjugation strategy for amplified magnetic labeling.
  • Application of the method to both surface and intracellular markers.
  • Demonstration in rare tumor cell isolation for cancer diagnosis.
  • Purification of chimeric antigen receptor (CAR) T cells for immunotherapy.

Main Results:

  • Achieved up to 100-fold amplification of magnetic labeling.
  • Successfully enabled high-performance magnetic cell sorting for rare cells.
  • Demonstrated utility in cancer diagnostics and CAR T cell therapy.
  • Showcased effective stratification of rare cell subpopulations.

Conclusions:

  • The developed method significantly enhances magnetic labeling efficiency.
  • This approach improves the performance of magnetic cell sorting for rare cell analysis.
  • The technique offers a valuable tool for cancer diagnosis and immunotherapy development.