Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

11.0K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
11.0K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.6K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.6K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.9K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.9K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.5K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.5K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

12.9K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
12.9K
Electron Behavior00:54

Electron Behavior

104.8K
Overview
Electrons are negatively charged subatomic particles that are attracted to an orbit around the positively-charged nucleus of an atom. They reside in locations that are associated with energy levels called shells and are further organized into sub-shells and orbitals within each shell.
Electrons Orbit the Nucleus
Electrons are found in specific locations outside of the nucleus. The shell in which an electron resides indicates the general energy level of the electron: those closer to the...
104.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Ahmed et al. Comparative Carcinogenicity of Double-Walled Carbon Nanotubes of Different Lengths Administered by Intratracheal Installation into Rat Lungs. <i>Nanomaterials</i> 2025, <i>15</i>, 1402.

Nanomaterials (Basel, Switzerland)·2026
Same author

Light- and chemical-induced ciliary signaling governs dorsal/ventral regionalization of human telencephalic organoids.

Nature communications·2026
Same author

Clinical performance of the multiplex solid-phase "Direct Strip PCR" for infectious uveitis: a multicenter diagnostic accuracy study.

Japanese journal of ophthalmology·2026
Same author

Molecular Variations in Glycoprotein B of Asian Human Cytomegalovirus: Potential Impact on Virus Entry and Immune Evasion in Ocular Diseases.

Journal of medical virology·2026
Same author

Mortality related to procedural sedation and analgesia: a 10-year review of a nationwide medical adverse events database.

BMJ open quality·2025
Same author

Clinical features and prognosis of immune checkpoint inhibitor-associated ocular inflammation in Japanese patients: a case series.

Japanese journal of ophthalmology·2025

Related Experiment Video

Updated: Sep 21, 2025

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology
09:55

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology

Published on: September 28, 2022

1.8K

A functional platform for remote use of electron microscopes using web conferencing systems.

Makoto Sugiura-Nakazato1,2, Hiroshi Takase3,4, Takeru Nakazato5

  • 1Section of Biostructural Science, Graduate School of Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-ku, Tokyo 113-8549, Japan.

Microscopy (Oxford, England)
|May 31, 2022
PubMed
Summary

We developed a system for remote transmission electron microscope (TEM) image sharing. Web conferencing tools like Teams, Zoom, and Google Meet were evaluated for clarity, smoothness, and lag, with virtual cameras and screen sharing as viable capture methods.

Keywords:
Open Broadcaster Softwareconference systemimage qualitymicroscopy imageremote sharingvirtual camera

More Related Videos

Single Particle Cryo-Electron Microscopy: From Sample to Structure
11:52

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

8.8K
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

34.7K

Related Experiment Videos

Last Updated: Sep 21, 2025

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology
09:55

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology

Published on: September 28, 2022

1.8K
Single Particle Cryo-Electron Microscopy: From Sample to Structure
11:52

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

8.8K
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

34.7K

Area of Science:

  • Materials Science
  • Microscopy
  • Computer Science

Background:

  • Remote collaboration is crucial for scientific research, but sharing high-resolution images from specialized equipment like transmission electron microscopes (TEMs) presents challenges.
  • Existing web conferencing tools offer potential solutions for remote image sharing, yet their performance with demanding applications like TEM imaging needs thorough evaluation.

Purpose of the Study:

  • To propose and evaluate a system for real-time remote sharing of transmission electron microscope (TEM) images.
  • To assess the performance of popular web conferencing platforms (Microsoft Teams, Zoom, Google Meet) for remote TEM image analysis.
  • To compare different image capture methods for optimizing remote TEM data transmission.

Main Methods:

  • A three-computer system was configured: one connected to the TEM, one for image distribution, and one for image reception.
  • Performance evaluation of Microsoft Teams, Zoom, and Google Meet based on image clarity, movement smoothness, and time lag.
  • Comparison of two image capture methods: virtual camera via video distribution software and direct screen sharing through conferencing software.

Main Results:

  • The proposed system enables remote sharing of TEM images between connected computers.
  • Performance varied across web conferencing systems, with differences noted in image clarity, smoothness, and time lag.
  • Virtual camera methods offered good reaction speed for image transfer, while screen sharing provided higher image quality.

Conclusions:

  • The developed system provides a functional framework for remote TEM image sharing.
  • Web conferencing platforms can be utilized for remote TEM analysis, but performance optimization is key.
  • The choice between virtual camera and screen sharing depends on the priority between speed and image fidelity for remote TEM applications.