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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

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Fluorescence Sensing Technologies for Ophthalmic Diagnosis.

Yuqi Shi¹, Yubing Hu¹, Nan Jiang²

¹Department of Chemical Engineering, Imperial College London, South Kensington, London, SW7 2BU, United Kingdom.

|May 31, 2022

Summary

Fluorescent contact lens sensors offer real-time ocular diagnostics by analyzing tear fluid for biomarkers. This point-of-care technology enhances disease monitoring and treatment effectiveness.

Keywords:

Contact lens sensors Fluorescence biosensing Fluorescence imaging Fluorescent tear diagnosis Ocular diseases Ophthalmological diagnostics Point-of-Care Smartphone readout devices Tear monitoring

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Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

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Area of Science:

Ophthalmic diagnostics
Biomedical engineering
Optical sensing technologies

Background:

Personalized and point-of-care (POC) diagnoses are crucial for ocular physiology and disease management.
Real-time tear fluid monitoring via user-friendly ophthalmic techniques is essential.
Fluorescence technologies are well-suited for clinical ophthalmic applications due to their sensing capabilities.

Purpose of the Study:

To review applied ophthalmic fluorescence sensing technologies in tear fluid for ocular diagnosis and monitoring.
To discuss the integration of fluorescent sensors with mobile devices for continuous ocular disease diagnosis.
To provide perspectives on future developments in fluorescent ocular sensing.

Main Methods:

Development of wearable contact lens sensors for fluorescence sensing in tear diagnostics.
Examination of various biochemical analytes in tears, including pH, electrolytes, glucose, and enzymes.
Integration of fabricated fluorescent sensors with mobile phone readout devices.

Main Results:

Fluorescent contact lens sensors enable real-time monitoring and continuous sampling of tear fluid.
These sensors have demonstrated effectiveness in examining multiple biochemical analytes for ocular health.
The technology supports POC settings for monitoring ocular conditions and treatment efficacy.

Conclusions:

Fluorescent contact lens sensors represent a significant advancement in ocular diagnostics.
The integration with mobile technology offers a promising avenue for continuous, personalized eye care.
Further development holds potential for improved diagnosis, treatment monitoring, and surgical tracking in ophthalmology.