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Updated: Sep 20, 2026

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
Published on: July 13, 2012
Influence of operational conditions on the performance of biogas bioconversion into ectoines in pilot bubble column
María Del Rosario Rodero1, Raquel Herrero-Lobo1, Víctor Pérez1
1Institute of Sustainable Processes, University of Valladolid, 47011, Valladolid, Spain; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineerings. University of Valladolid, Dr. Mergelina s/n., 47011, Valladolid, Spain.
Abstract:
The application of biogas as a low-priced substrate for the production of ectoines constitutes an opportunity to decrease their production costs and to enhance the viability of anaerobic digestion. The influence of operational conditions on CH4-biogas biodegradation and on ectoines production yields was assessed in continuous pilot bubble column bioreactors. The rise in biomass concentration from 1 to 3 g L-1 resulted in a decrease in the specific ectoine content from 42 ± 8 to 30 ± 4 mgectoine gVSS-1. The concentration of Cu2+ and Mg2+ did not impact process performance, while the use of ammonium as N source resulted in low CH4 biodegradation and ectoine yields (13 ± 7 mgectoine gVSS-1). The increase in CH4 content from 4.5 to 9 %v·v-1 enhanced CH4 removal efficiency. Process operation at NaCl concentrations of 3 %w·w-1 instead of 6 %w·w-1 decreased the ectoine yield to 17 mgectoine gVSS-1. Finally, Methylomicrobiumburyatense was identified as the dominant species.
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