Related Experiment Video
Updated: Sep 21, 2025

Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
Genotyping Platforms for Genome-Wide Association Studies: Options and Practical Considerations
1Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA. david.hyten@unl.edu.
Selecting the right genotyping technology is crucial for cost-effective, high-density data in crop genome-wide association studies (GWAS). Various platforms offer options for different crop genomes and research goals.
Area of Science:
- Agricultural Science
- Genomics
- Plant Breeding
Background:
- Genome-wide association studies (GWAS) in crops necessitate efficient, high-density genotyping.
- Current commercial platforms and resequencing methods are widely employed for crop genotyping.
Purpose of the Study:
- To review available genotyping platforms for crop GWAS.
- To discuss considerations for selecting appropriate genotyping technologies based on crop and study objectives.
Main Methods:
- Genotyping arrays (Illumina Infinium, Applied Biosystems Axiom) and resequencing methods are utilized.
- These methods genotype tens of thousands to millions of markers.
Main Results:
- Platforms are effective across diverse crop genomes, from simple to complex and polyploid.
- Successful genotyping of large GWAS panels is achievable.
Conclusions:
- Choosing the correct genotyping technology is vital for achieving desired coverage, accuracy, and cost-effectiveness in crop GWAS.
- Practical considerations are key for successful implementation.
More Related Videos
05:53Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
11:35Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
Published on: August 21, 2016
Related Concept Videos
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
Genomics
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
DNA Microarrays