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Published on: August 26, 2009
Controlled nano-agglomerates as stabile SERS reporters for unequivocal labelling
Can Xiao1, Bernat Mir-Simón2, Pilar Rivera-Gil3
1Integrative Biomedical Materials and Nanomedicine Lab, Department of Medicine and Life Sciences, University Pompeu Fabra, Doctor Aiguader 88, 08003, Barcelona, Spain.
Abstract:
Biosensors, especially those with a SERS readout, are required for an early and precise healthcare diagnosis. Unreproducible SERS platforms hamper clinical SERS. Here we report a synthetic procedure to obtain stabile, reproducible and robust highly-SERS performing nanocomposites for labelling. We controlled the NPs agglomeration and codification which resulted in an increased number of hot spots, thus exhibiting reproducible and superior Raman enhancement. We studied fundamental aspects affecting the plasmonic thiol bond resulting in pH exhibiting a determining role. We validated their biosensing performance by designing a SERS-based detection assay model for SARS-CoV-2. The limit of detection of our assay detecting the spike RBD was below 10 ng/mL.
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