Related Experiment Video

Updated: Sep 21, 2025

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
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Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

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RNA m6A reader YTHDF1 facilitates inflammation via enhancing NLRP3 translation

Wei-Yuan Hao1, Yan Lou2, Guo-Ying Hu3

  • 1Department of Intervention, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, 1 East Banshan Road, Hangzhou, 310022, Zhejiang, China.

Biochemical and Biophysical Research Communications
|June 1, 2022
PubMed

Insights

YTHDF1, an RNA m6A reader, promotes inflammatory cytokine IL-1β production during bacterial infections. Inhibiting YTHDF1 improves survival in sepsis models, suggesting it as a therapeutic target for inflammatory diseases.

Area of Science:

  • Molecular Biology
  • Immunology
  • Epigenetics

Background:

  • N6-methyladenosine (m6A) mRNA modification is crucial for biological processes.
  • m6A regulation involves "writers", "erasers", and "readers".
  • Functional roles of m6A reader proteins, like YTHDFs, remain largely unclear.

Purpose of the Study:

  • To elucidate the role of the RNA m6A reader protein YTHDF1 in inflammatory responses.
  • To investigate YTHDF1's involvement in macrophage pro-inflammatory cytokine production.
  • To assess YTHDF1 as a potential therapeutic target for inflammatory diseases.

Main Methods:

  • Investigated YTHDF1 function in macrophages during bacterial infection models.
  • Analyzed the effect of YTHDF1 overexpression on NLRP3 translation.
  • Utilized in vivo knockdown of YTHDF1 in a mouse model of sepsis.

Main Results:

  • YTHDF1 was found to promote pro-inflammatory IL-1β production in macrophages.
  • Overexpression of YTHDF1 enhanced NLRP3 translation.
  • In vivo knockdown of YTHDF1 significantly improved survival rates in a mouse sepsis model.

Conclusions:

  • YTHDF1 plays a significant role in mediating inflammatory responses and subsequent tissue injury.
  • YTHDF1 is a key mediator of IL-1β production during bacterial infections.
  • YTHDF1 represents a promising therapeutic target for managing inflammatory diseases and sepsis.
Keywords:
InflammationN(6)-methyladenosineNLRP3YTHDF1

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