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Updated: Sep 21, 2025
Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
Comparative study of Pd@Pt nanozyme improved colorimetric N-ELISA for the paper-output portable detection of
Xin Wang1, Min Zhang1, Xiaoxu Pang1
1Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
Abstract:
Staphylococcus aureus is a frequent pathogenic bacterium that has a significant detrimental influence on the health of the human body. Therefore, developing a practical and portable detection platform is critical to ensuring food safety. Nanozymes are a kind of engineered nanomaterials with superior enzyme-like activities, providing infinite possibilities for the development of highly sensitive analytical assays. In this study, mesoporous core-shell palladium@platinum (Pd@Pt) nanozymes were synthesized and then applied as a signal amplifier in Staphylococcus aureus colorimetric immunoassay. At the same time, a careful comparative study of the catalytic performance with natural horseradish peroxidase (HRP), Pd@Pt nanozymes and their complexes Pd@Pt-HRP (HRP coupling with Pd@Pt nanozymes) were firstly performed, as well as clever using a colorimeter to achieve portable signal output. Pd@Pt-HRP bioprobes enable remarkable peroxidase-like catalytic activity, resulting in the highest sensitivity with a limit of detection (LOD) improved from 1 × 105 to 1 × 103 CFU/mL. In all, this work conducted a valuable comparative study and presented an improved strategy for the portable detection of pathogenic bacteria, which was expected to be used in a wide range of applications in food safety regulation and biomedicals arenas.
