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Updated: Sep 21, 2025
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Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
Published on: September 8, 2009
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In vivo molecular and single cell imaging.
Seongje Hong1, Siyeon Rhee2, Kyung Oh Jung3
1Department of Anatomy, College of Medicine, Chung-Ang University, Seoul 06974, Korea.
BMB Reports
|June 2, 2022
Summary
Molecular imaging advances disease diagnosis and treatment monitoring. Recent developments enable in vivo single cell imaging, offering unprecedented insights into cellular behavior and therapeutic effectiveness.
Area of Science:
- Biomedical imaging
- Molecular biology
- Cellular biology
Background:
- Molecular imaging enhances disease diagnosis, prognosis, and treatment monitoring in living subjects.
- Various molecular targets and imaging modalities (Optical, MRI, PET, SPECT, CT) visualize in vivo biological processes.
- In vivo cell imaging is crucial for monitoring cell migration, activity, and therapeutic effects, particularly in cancer and cell-based therapies.
Approach:
- Reviewing the basic principles of in vivo molecular imaging.
- Introducing recent advancements in in vivo single cell imaging techniques.
- Highlighting the application of molecular imaging for tracking labeled cells (cancer, immune, stem cells) in vivo.
Key Points:
- Molecular imaging visualizes anatomic, genetic, biochemical, and physiologic changes.
- In vivo cell imaging tracks cell migration, activity, and therapeutic outcomes.
- Single cell imaging in vivo presents challenges but is crucial for understanding individual cell behavior.
Conclusions:
- In vivo molecular imaging is a powerful tool for disease management and therapy monitoring.
- Recent progress in single cell imaging opens new avenues for detailed cellular analysis in live subjects.
- The review covers fundamental principles and emerging studies in in vivo single cell imaging.
