Co-Expression of TIGIT and Helios Marks Immunosenescent CD8⁺ T Cells During Aging

Aging leads to alterations in the immune system that result in ineffective responsiveness against pathogens. Features of this process, collectively known as immunosenescence, accumulate in CD8⁺ T cells with age and have been ascribed to differentiation of these cells during the course of life. Here we aimed to identify novel markers in CD8⁺ T cells associated with immunosenescence. Furthermore, we assessed how these markers relate to the aging-related accumulation of highly differentiated CD27^-CD28^- cells. We found that co-expression of the transcription factor Helios and the aging-related marker TIGIT identifies CD8⁺ T cells that fail to proliferate and show impaired induction of activation markers CD69 and CD25 in response to stimulation in vitro. Despite this, in blood of older adults we found TIGIT⁺Helios⁺ T cells to become highly activated during an influenza-A virus infection, but these higher frequencies of activated TIGIT⁺Helios⁺ T cells associate with longer duration of coughing. Moreover, in healthy individuals, we found that TIGIT⁺Helios⁺ CD8⁺ T cells accumulate with age in the highly differentiated CD27^-CD28^- population. Interestingly, TIGIT⁺Helios⁺ CD8⁺ T cells also accumulate with age among the less differentiated CD27⁺CD28^- T cells before their transit into the highly differentiated CD27^-CD28^- stage. This finding suggests that T cells with immunosenescent features become prominent at old age also within the earlier differentiation states of these cells. Our findings show that co-expression of TIGIT and Helios refines the definition of immunosenescent CD8⁺ T cells and challenge the current dogma of late differentiation stage as proxy for T-cell immunosenescence.