Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

311
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
311

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blinking-Based Identification of Single Dye Molecules in Ink.

Analytical chemistry·2026
Same author

Uncovering Art's Vanishing Hues with Surface-Enhanced Raman Scattering: Drawing Inspiration from the Past for the Future.

ACS nano·2024
Same author

Blinking characteristics of organic fluorophores for blink-based multiplexing.

Communications chemistry·2024
Same author

Rapid, Accurate Classification of Single Emitters in Various Conditions and Environments for Blinking-Based Multiplexing.

The journal of physical chemistry. A·2023
Same author

Unraveling the Excited-State Dynamics of Eosin Y Photosensitizers Using Single-Molecule Spectroscopy.

The journal of physical chemistry. A·2019

Related Experiment Video

Updated: Sep 21, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.0K

Blinking-Based Multiplexing: A New Approach for Differentiating Spectrally Overlapped Emitters.

Grace A DeSalvo1, Grayson R Hoy1, Isabelle M Kogan1

  • 1Department of Chemistry, William & Mary, P.O. Box 8795, Williamsburg, Virginia 23187, United States.

The Journal of Physical Chemistry Letters
|June 2, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed blinking-based multiplexing (BBM) to distinguish fluorescent molecules by their blinking patterns, not just color. This simple method improves multicolor single-molecule imaging accuracy without needing spectrally distinct probes.

More Related Videos

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.1K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K

Related Experiment Videos

Last Updated: Sep 21, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.0K
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.1K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K

Area of Science:

  • Single-molecule imaging
  • Spectroscopy
  • Biophysics
  • Materials Science

Background:

  • Multicolor single-molecule imaging is crucial for biological and materials science research.
  • Current multiplexing methods rely on spectrally distinct probes or sequential imaging, which have limitations.

Purpose of the Study:

  • To introduce a novel method, blinking-based multiplexing (BBM), for differentiating spectrally overlapped emitters.
  • To demonstrate that emitters can be classified based on intrinsic blinking dynamics, independent of spectral color.

Main Methods:

  • Acquired blinking dynamics of rhodamine 6G and CdSe/ZnS quantum dots under identical settings.
  • Analyzed blinking data using a change point detection algorithm and a deep learning algorithm for classification.

Main Results:

  • A blinking metric achieved 93.5% classification accuracy for differentiating emitters.
  • Deep learning classification further improved accuracy to 96.6%.
  • Demonstrated accurate emitter classification based solely on blinking dynamics.

Conclusions:

  • Blinking-based multiplexing (BBM) offers a simple and effective approach for multicolor single-molecule imaging.
  • BBM overcomes limitations of spectral overlap by utilizing intrinsic blinking heterogeneity.
  • This technique enhances multiplexing capabilities without requiring spectrally distinct fluorescent probes.