Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Uncovering Art's Vanishing Hues with Surface-Enhanced Raman Scattering: Drawing Inspiration from the Past for the Future.

ACS nano·2024

Same author

Blinking characteristics of organic fluorophores for blink-based multiplexing.

Communications chemistry·2024

Same author

Rapid, Accurate Classification of Single Emitters in Various Conditions and Environments for Blinking-Based Multiplexing.

The journal of physical chemistry. A·2023

Same author

Blinking-Based Multiplexing: A New Approach for Differentiating Spectrally Overlapped Emitters.

The journal of physical chemistry letters·2022

Same author

Unraveling the Excited-State Dynamics of Eosin Y Photosensitizers Using Single-Molecule Spectroscopy.

The journal of physical chemistry. A·2019

Search research articles

Related Experiment Video

Updated: Jun 14, 2026

Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks

Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks

Published on: May 8, 2015

Blinking-Based Identification of Single Dye Molecules in Ink.

Alisha J Khodabocus¹, Walker T Knapp¹, Benjamin T Steinman¹

¹Department of Chemistry, William & Mary, PO Box 8795, Williamsburg, Virginia 23187-8795, United States.

Analytical Chemistry

|January 2, 2026

Summary

This summary is machine-generated.

This study introduces a novel blinking-based method for identifying single fluorescent dye molecules in cultural heritage materials. This ultrasensitive technique offers exclusive identification of rhodamine dyes without invasive substrates.

More Related Videos

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

Published on: May 22, 2019

Related Experiment Videos

Last Updated: Jun 14, 2026

Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks

Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks

Published on: May 8, 2015

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

Published on: May 22, 2019

Area of Science:

Analytical Chemistry
Heritage Science
Materials Science

Background:

Identifying fluorescent dyes in cultural heritage is difficult due to fading, sample scarcity, and complexity.
Existing methods like surface-enhanced Raman scattering (SERS) can be invasive or lack single-molecule sensitivity.

Purpose of the Study:

To develop a minimally invasive, ultrasensitive method for identifying single fluorescent dye molecules.
To provide exclusive identification of structurally similar rhodamine dyes in inks.

Main Methods:

Utilized widefield fluorescence microscopy and blinking analysis to detect intrinsic fluorescence intermittency.
Applied change point detection and multinomial logistic regression to define identification factors.
Validated results with bulk surface-enhanced Raman scattering (SERS) measurements.

Main Results:

Successfully identified single rhodamine dye molecules based on their unique blinking patterns.
Developed four quantitative determination factors for exclusive identification of three rhodamine dyes.
Confirmed the presence of rhodamine B in commercial ballpoint ink samples.

Conclusions:

Blinking-based dye identification offers single-molecule sensitivity and exclusive results without plasmonic substrates.
This method is a powerful, minimally invasive tool for characterizing artists' materials.
Opens new avenues for conservation and heritage science research.