Related Experiment Video
Updated: Sep 21, 2025

Monitoring Plant Hormones During Stress Responses
Published on: June 15, 2009
Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds
Caroline Lelarge-Trouverie1, Amna Mhamdi2, Florence Guérard3
1Institut des Sciences des Plantes de Paris-Saclay, Unité Mixte de Recherche 8618 Centre National de la Recherche Scientifique, Orsay Cedex, France.
Abstract:
Measuring quantitative changes in plant hormones and derivatives is crucial to understand how reactive oxygen species trigger signaling cascades to regulate stress responses. In this chapter, we describe the liquid chromatography-mass spectrometry procedure that we use to extract and quantify salicylic acid (SA), jasmonic acid (JA), and related compounds in common extracts of Arabidopsis tissue. The method can provide quantitative data on SA, SA glucosides, and JA, as well as information on oxidized and conjugated forms of these compounds and related derivatives of benzoic acid.
Related Concept Videos
Responses to Heat and Cold Stress
Plant Hormones
Defenses Against Pathogens and Herbivores
Responses to Salt Stress
Physiological Foundation of Stress
Role of the Sympathetic Nervous System
Adrenaline triggers the...

