Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

6.0K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
6.0K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

7.0K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Humans and rhesus macaques share maturation pathways of HIV-1 envelope-reactive V3-glycan bnAb lineages.

Science translational medicine·2026
Same author

Enhanced Human Antigen-Specific B Cell Responses Using In Vitro 3D Tonsil Cultures Containing Stromal Cells.

Advanced healthcare materials·2026
Same author

Humoral and cellular immune response after COVID-19 vaccination in patients with sickle cell disease on hydroxyurea.

Blood advances·2026
Same author

Identification of a potent V3 glycan site broadly neutralizing antibody targeting an N332<sub>gp120</sub> glycan-independent epitope.

Nature immunology·2026
Same author

Humoral and cellular immune response after COVID-19 vaccination in patients with sickle cell disease on hydroxyurea.

Blood advances·2025
Same author

HIV-1 envelope trimer vaccine induces sex-associated differences in antibody responses: a phase 1 clinical trial.

Nature communications·2025

Related Experiment Video

Updated: Sep 21, 2025

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
11:12

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Published on: April 11, 2019

7.2K

From affinity selection to kinetic selection in Germinal Centre modelling.

Danial Lashgari1, Elena Merino Tejero1, Michael Meyer-Hermann2,3

  • 1Bioinformatics Laboratory, Epidemiology and Data Science, Amsterdam Public Health research institute, Amsterdam Institute for Infection and Immunity, Amsterdam, the Netherlands.

Plos Computational Biology
|June 3, 2022
PubMed
Summary

Germinal center selection favors B cells with lower dissociation rates, enhancing antibody affinity maturation. This kinetic bias, driven by probabilistic bond rupture, influences antibody evolution and immune memory formation.

More Related Videos

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
09:35

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Published on: April 20, 2021

6.9K
Examination of Thymic Positive and Negative Selection by Flow Cytometry
14:29

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Published on: October 8, 2012

22.1K

Related Experiment Videos

Last Updated: Sep 21, 2025

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
11:12

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Published on: April 11, 2019

7.2K
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
09:35

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Published on: April 20, 2021

6.9K
Examination of Thymic Positive and Negative Selection by Flow Cytometry
14:29

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Published on: October 8, 2012

22.1K

Area of Science:

  • Immunology
  • Computational Biology
  • Evolutionary Biology

Background:

  • Affinity maturation increases antibody affinity through B-cell evolution in germinal centers (GCs).
  • Mechanisms of B-cell receptor (BcR) affinity discrimination and selection in GCs are not fully understood.
  • GC selection may favor both rapid (kinetic) and tight (thermodynamic) binding.

Purpose of the Study:

  • To investigate potential GC selection biases towards rapid and tight B-cell receptor binding.
  • To understand how antigen collection mechanisms influence affinity maturation dynamics.
  • To compare affinity-based vs. kinetic-based antigen collection in silico.

Main Methods:

  • Developed an agent-based model of the germinal center.
  • Simulated B-cell evolution with varying association/dissociation rates.
  • Compared three antigen collection mechanisms: affinity-based (Scenario-0), kinetic-based with bond rupture (Scenario-1), and kinetic-based without bond rupture (Scenario-2).

Main Results:

  • Antigen collection mechanisms significantly impact GC dynamics and B-cell binding kinetics.
  • In Scenario-1, B-cell clones with lower dissociation rates outcompeted those with higher association rates.
  • Plasma cell and memory B cell populations showed a bias towards clones with lower dissociation rates.
  • Removing probabilistic bond rupture (Scenario-2) reduced the advantage of low dissociation rates and decreased overall affinity maturation.

Conclusions:

  • Germinal center selection can exhibit a bias towards kinetic properties, specifically lower dissociation rates.
  • Probabilistic rupture of B-cell-antigen bonds during antigen collection is a key factor driving this kinetic selection bias.
  • This bias influences the evolution of antibody affinity and the composition of antibody-producing and memory B-cell populations.