Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automatic identification of diagnosis from hospital discharge letters via weakly supervised Natural Language Processing.

Scientific reports·2026
Same author

Effects of antibiotic treatment on clinical outcomes in patients with COVID-19: a systematic review and meta-analysis.

The Lancet. Microbe·2026
Same author

Paediatric HIV cure section in Current Opinion in HIV and AIDS.

Current opinion in HIV and AIDS·2026
Same author

Dolutegravir as First- or Second-line Treatment for Children With HIV: 240-Week Follow-up of the ODYSSEY Randomized Trial.

Open forum infectious diseases·2026
Same author

Burden of non-invasive fungal infections and antifungal therapy in pediatric primary care settings: FUNGICARE Project.

Italian journal of pediatrics·2026
Same author

Nirsevimab and a Community Recall-Based Immunization Strategy for Child Bronchiolitis Prevention.

JAMA network open·2026

Related Experiment Video

Updated: Sep 21, 2025

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
13:58

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Published on: September 26, 2011

31.8K

Assessing the Variability of Cell-Associated HIV DNA Quantification through a Multicenter Collaborative Study.

Yann Le Duff1, Kathleen Gärtner2, Eloise J Busby3

  • 1Division of Infectious Disease Diagnostics, Centre for AIDS Reagent, National Institute for Biological Standards and Control, South Mimms, United Kingdom.

Microbiology Spectrum
|June 6, 2022
PubMed
Summary

Quantifying cell-associated HIV DNA (CA HIV DNA) shows significant variability across different assays. Carefully selected reference materials can harmonize these measurements, improving HIV diagnosis and patient management.

Keywords:
HIV DNAcollaborative studydiagnosticshuman immunodeficiency virusquantification variabilityreference materials

More Related Videos

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings
19:57

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Published on: March 30, 2014

18.7K
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

13.9K

Related Experiment Videos

Last Updated: Sep 21, 2025

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
13:58

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Published on: September 26, 2011

31.8K
An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings
19:57

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Published on: March 30, 2014

18.7K
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

13.9K

Area of Science:

  • Virology
  • Molecular Diagnostics
  • Public Health

Background:

  • Accurate quantification of cell-associated HIV DNA (CA HIV DNA) is crucial for diagnosing HIV in infants, managing patients on therapy, and evaluating new treatments.
  • HIV remains a significant global health challenge, with millions of new infections annually.

Purpose of the Study:

  • To assess the variability in CA HIV DNA quantification across different diagnostic assays.
  • To evaluate the effectiveness of reference materials in harmonizing CA HIV DNA measurements.

Main Methods:

  • A multicenter collaborative study was conducted using a common set of reagents.
  • Variability was assessed across various assays for CA HIV DNA quantification.
  • Cross-subtype normalization and calibration with the 8E5 cell line were explored.

Main Results:

  • Significant variability in CA HIV DNA quantification and lower limit of quantification was observed across assays.
  • Cross-subtype normalization helped harmonize CA HIV DNA quantification.
  • Calibration with the 8E5 cell line did not reduce inter-assay variability.

Conclusions:

  • Assay, protocol, and reagent heterogeneity contribute to significant variability in HIV DNA measurements between laboratories.
  • Carefully selected reference materials can reduce measurement variability and harmonize CA HIV DNA quantification.
  • Improved comparability of diagnostic assay performance is needed for better HIV diagnosis and patient management.