Estradiol binding capacity of breast cancer cell line in correlation with cell cycle

The specific estrogen binding capacity of the MCF-7 cells has been studied throughout the cell cycle. Two days after plating 1-4 X 10(5) cells per well, we observed that cell number, protein concentration and DNA level began to rise but the estrogen binding capacity was lowered. At logarithmic phase the binding increased gradually then sharply to just before confluency. When the cells reached the quiescent phase the estrogen binding capacity fell rapidly. Since estrogen binding capacity of the intact MCF-7 cells varies with different phases in the cell cycle as well as the incubation temperature and time and the plating density, all these factors should be taken into consideration when this cell line is used for hormone studies.