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Updated: Sep 20, 2025

Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Mass Spectrometry for the Analysis of Highly Charged Sulfated Carbohydrates
Lianli Chi1, Jonathan Amster2, Robert J Linhardt1
1Department of Chemistry and Chemical Biology, Chemical and Biological Engineering, and Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Abstract:
Sulfated polysaccharides and their oligosaccharide components play an important biological role in physiological and pathophysiological processes. Glycosaminoglycans, such as heparin, are currently used as pharmaceutical agents to control coagulation. Moreover, there arc intensive efforts in developing rapid approaches for the analysis of such bioactive carbohydrates to assess their pharmacological activity and in glycomic applications. Soft ionization methods of mass spectrometry have been applied with recent success in the analysis of sulfated carbohydrates. This review will examine the application of mass spectrometry in the compositional analysis and structural characterization of these important highly charged molecules.
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