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Updated: Sep 20, 2025

Author Spotlight: Unraveling the Mysteries of Terrestrial Anaerobic Microorganisms in Uncharted Environments by In Situ Culturing
Published on: January 12, 2024
Hymenobacter translucens sp. nov. and Hymenobacter pini sp. nov. isolated from soil
Minji Bang1, Myung Kyum Kim2, Hee-Young Jung3,4
1Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 01797, Republic of Korea.
Abstract:
Two novel bacterial strains BT175T and BT728T were isolated from soil collected in the Republic of Korea. Both strains were Gram stain negative, rod shaped, and had circular, convex, and pink-colored colonies. The 16S rRNA gene sequence similarity between the strains BT175T and BT728T was 93.6%, indicating that they represent a distinct species. 16S rRNA sequence analysis indicated that strains BT175T and BT728T belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia, phylum Bacteroidetes). Strain BT175T was closely related to Hymenobacter persicinus 1-3-3-3T (97.2%, 16S rRNA gene similarity), Hymenobacter knuensis 16F7C-2T (96.6%), and Hymenobacter daejeonensis PB105T (96.1%). Strain BT728T was closely related to Hymenobacter rigui KCTC 12533T (98.4%), Hymenobacter metallilatus 9PBR-2T (97.6%), and Hymenobacter perfusus LMG26000T (97.4%). Strain BT175T and BT728T were found to have the MK-7 as the major respiratory quinone. The DNA G + C content of strain BT175T was 61.6 mol% and BT728T was 59.6 mol%, respectively. Characterization based on polyphasic analysis indicated that strains BT175T and BT728T represent novel species of the genus Hymenobacter and the names Hymenobacter translucens sp. nov. and Hymenobacter pini sp. nov. are proposed. The type strain of Hymenobacter translucens is BT175T (= KCTC 72330T = NBRC 115441T) and Hymenobacter pini is BT728T (= KACC 22629T = NBRC 115444T).
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