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Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells
Hana Kotasová1,2, Michaela Capandová1, Vendula Pelková1
1Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
Tissue Engineering and Regenerative Medicine
|June 7, 2022
Summary
Human embryonic stem cells (hESC) can be differentiated into expandable lung epithelium (ELEP). These ELEP are a scalable cell source for lung research and therapies.
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Pulmonary Biology
Background:
- Lung epithelial progenitors are valuable for cell-based therapies, disease modeling, and toxicity testing.
- Obtaining lung progenitors involves direct isolation or in vitro differentiation.
- Pluripotent stem cells offer a promising source for autologous and allogeneic lung cells.
Purpose of the Study:
- To derive expandable lung epithelium (ELEP) from human embryonic stem cells (hESC).
- To establish culture conditions for long-term propagation of hESC-derived ELEP.
Main Methods:
- Utilized the differentiation potential of hESC.
- Established monolayer culture conditions for propagation exceeding 6 months.
- Avoided 3D culture and cell sorting to minimize variability.
Main Results:
- Successfully derived expandable lung epithelium (ELEP) from hESC.
- ELEP express NKX2.1, a marker of early lung lineage.
- ELEP exhibit properties of early surfactant production and differentiate into airway and alveolar epithelial cells.
Conclusions:
- hESC-derived ELEP provide a stable, scalable, and high-yielding cell source.
- ELEP are suitable for various biomedical applications.
- This method offers a convenient and consistent approach to generating lung epithelial cells.
