Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Shape transformations induced by amphiphiles in erythrocytes.

B Isomaa, H Hägerstrand, G Paatero

    Biochimica Et Biophysica Acta
    |May 12, 1987
    PubMed
    Summary

    Amphiphiles alter red blood cell shape, forming echinocytes or stomatocytes. These shape changes, induced by various amphiphile types, suggest a mechanism beyond simple lipid bilayer intercalation, involving intrabilayer non-bilayer phase formation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of the Choice of Primary Chemotherapy on the Cumulative Length of Stay in Hospital for Multiple Myeloma.

    Leukemia & lymphoma·2016
    Same author

    MEIC Evaluation of Acute Systemic Toxicity: Part VI. The Prediction of Human Toxicity by Rodent LD50 Values and Results From 61 In Vitro Methods.

    Alternatives to laboratory animals : ATLA·2015
    Same author

    Generation of a neutral FST baseline for testing local adaptation on gill raker number within and between European whitefish ecotypes in the Baltic Sea basin.

    Journal of evolutionary biology·2015
    Same author

    Gill epithelial cells as in vitro models in aquatic toxicology.

    Alternatives to laboratory animals : ATLA·2014
    Same author

    The human L-type calcium channel Cav1.3 regulates insulin release and polymorphisms in CACNA1D associate with type 2 diabetes.

    Diabetologia·2012
    Same author

    Family history of Type 1 diabetes affects insulin secretion in patients with 'Type 2' diabetes.

    Diabetic medicine : a journal of the British Diabetic Association·2012

    Area of Science:

    • Biochemistry and Biophysics
    • Cell Biology
    • Membrane Biophysics

    Background:

    • Amphiphiles are molecules with both hydrophilic and hydrophobic properties.
    • They can interact with cell membranes, potentially altering cell shape.
    • Previous hypotheses, like the bilayer couple hypothesis, suggested intercalation explains shape changes.

    Purpose of the Study:

    • To investigate shape alterations in human erythrocytes induced by various amphiphiles.
    • To compare the effects of cationic, anionic, zwitterionic, and nonionic amphiphiles.
    • To test the validity of the bilayer couple hypothesis in explaining amphiphile-induced erythrocyte shape changes.

    Main Methods:

    • Studied shape changes in human erythrocytes treated with different amphiphiles (C10-C16) at specific concentrations.

    Related Experiment Videos

  • Observed effects of anionic, zwitterionic, nonionic, and cationic amphiphiles on erythrocyte morphology.
  • Assessed time-dependence of shape alterations and reversibility after amphiphile removal.

    • Main Results:

    • Anionic and zwitterionic amphiphiles were potent echinocytogenic agents (inducing crenation).
    • Nonionic amphiphiles showed varied effects, including stomatocytogenesis (inducing cup shapes) and echinocytogenesis.
    • Cationic amphiphiles induced time-dependent shape changes, initially crenation, then shifting towards stomatocytes.

    Conclusions:

    • Amphiphile-induced erythrocyte shape changes cannot be solely explained by the bilayer couple hypothesis.
    • A novel mechanism involving the formation of intrabilayer non-bilayer phases is proposed.
    • This phase formation likely facilitates transbilayer redistribution of amphiphiles and lipids, stabilizing the membrane.