Hybridoma Screening by Antibody Capture: Immunohistochemistry
Cold Spring Harbor Protocols
|June 7, 2022
Abstract:
To determine the subcellular location of an antigen, hybridoma tissue culture supernatants can be screened using immunohistochemistry. For antibodies to have access to antigens in fixed and embedded tissue sections, the paraffin must be removed and the tissue must be rehydrated and digested before immunohistochemical staining.
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