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Updated: Sep 20, 2025

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
Reproduction of Meloidogyne javanica in soybean genotypes
Ricardo R Balardin1, Cristiano Bellé1,2, Bruno C Piovesan1
1Universidade Federal de Santa Maria, Avenida Roraima, 1000, Bairro Camobi, 97105-900 Santa Maria, RS, Brazil.
Abstract:
Meloidogyne javanica is among the most important nematodes that damage soybean, and although genetic resistance is the ideal control measure, there are few cultivars described as resistant among those recommended for southern Brazil. The objective of this work was to evaluate the reaction of soybean cultivars to M. javanica. The inoculum of nematodes (Est. J3) was obtained from soybean plants and inoculated into tomato plants cultivar "Santa Cruz". Thirty-seven soybean cultivars widely used in the South, Southeast and Midwest of Brazil were used in the experiment. For each plant a suspension of 5,000 eggs + juveniles of second stage of M. javanica was inoculated into a sterile soil hole in 2-liter pots with six replications. The evaluation of root weight, number of galls, number of nematodes was 60 days after M. javanica inoculation. The results were subjected to analysis of variance, and the averages of each treatment were compared to each other by the Scott-Knott cluster test at 5% probability. Even though M. javanica presented RF> 1.00 in all soybean genotypes tested, different levels of susceptibility were observed. Thus, the lowest reproduction of the root-knot nematode was observed in M 5947 IPRO, HO AMAMBAY IPRO, BMX GARRA IPRO and FPS ATALANTA.
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