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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
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Related Experiment Video

Updated: Sep 20, 2025

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
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Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads.

Takamasa Mizoguchi1, Hikaru Handa1, Shuhei Omaru1

  • 1Graduate School of Pharmaceutical Science, Chiba University, Chiba, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|June 8, 2022
PubMed
Summary

Notch signaling activation requires cell contact and force. Immobilized ligand beads provide an efficient method for transient Notch signaling activation in cell culture, allowing controlled timing.

Keywords:
ImmobilizedLigand beadsLuciferase reporter assayNotch signaling

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Area of Science:

  • Cell biology
  • Molecular signaling

Background:

  • Notch signaling is crucial for cell-cell communication and development.
  • Activation necessitates physical cell-cell interaction and mechanical force for ligand-receptor engagement and cleavage.
  • Soluble ligands are insufficient for effective Notch activation in vitro.

Purpose of the Study:

  • To develop an efficient method for transiently activating Notch signaling in cell culture.
  • To overcome limitations of using soluble recombinant Notch ligands.

Main Methods:

  • Immobilization of Notch ligands onto beads.
  • Utilizing these beads for cell culture experiments to activate Notch signaling.
  • Controlling the timing of Notch signaling activation.

Main Results:

  • Demonstrated efficient transient activation of Notch signaling using immobilized ligand beads.
  • Showcased the ability to precisely control the timing of Notch pathway activation.
  • Established a novel method for studying Notch signaling dynamics.

Conclusions:

  • Immobilized ligand beads offer a robust and controllable system for Notch signaling activation.
  • This method facilitates the study of Notch signaling dynamics in various cell-based assays.
  • Provides a valuable tool for researchers investigating Notch-dependent biological processes.