Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster

The Notch (N) signaling pathway plays versatile roles in development and disease of many model organisms (Andersson et al., Development 138, 3593-3612, 2011; Hori et al., J Cell Sci, 126, 2135-2140, 2013). However, studying a role for N in adult tissue of Drosophila melanogaster is challenging, because the gene is located on the X-chromosome. To reduce the expression of N specifically in either the germline or the somatic cells of the adult gonad, we used the CRISPR technology in combination with the UAS/Gal4 expression system. After generation of the flies, gonads were investigated for germline survival. Here, we outline our detailed protocol.