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Candida auris Identification and Profiling by MALDI-ToF Mass Spectrometry
Alireza Abdolrasouli1,2, Mark A Fraser3
1Department of Medical Microbiology, King's College Hospital , London, UK. alireza.abdolrasouli@nhs.net.
Methods in Molecular Biology (Clifton, N.J.)
|June 8, 2022
Summary
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) is a standard for identifying yeasts in clinical labs. This chapter reviews protocols for identifying Candida auris using MALDI-ToF MS.
Area of Science:
- Microbiology
- Mycology
- Proteomics
Background:
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) is the standard for yeast identification in clinical laboratories.
- It has largely replaced traditional phenotypic methods due to its high throughput, accuracy, and cost-effectiveness.
- Specialized or in-house databases enhance MALDI-ToF MS capabilities for fungal identification.
Purpose of the Study:
- To review standard protocols for proteomic-based identification of Candida auris.
- To provide guidance for diagnostic and research laboratories handling clinical or environmental samples.
Main Methods:
- Review of three standard protocols for proteomic-based identification.
- Utilizing MALDI-ToF MS for fungal identification.
- Analysis of Candida auris isolates from various sample types.
Main Results:
- MALDI-ToF MS offers a high-throughput, accurate, and cost-effective method for yeast identification.
- Standardized workflows facilitate identification even in labs with limited expertise.
- The reviewed protocols are suitable for identifying Candida auris.
Conclusions:
- MALDI-ToF MS is an ideal platform for routine clinical, diagnostic, and research microbiology.
- Effective identification of pathogenic fungi like Candida auris is achievable with established protocols.
- The chapter provides valuable insights into Candida auris identification using proteomic approaches.