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Cell Viability Assays for Candida auris.

Jason L Brown1,2, Bryn Short3,4, Alicia Ware4,5

  • 1Oral Sciences Research Group, College of Medical, Veterinary and Life Sciences, Glasgow University, Glasgow, UK. jason.brown@glasgow.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|June 8, 2022
PubMed
Summary
This summary is machine-generated.

This study details methods for measuring the viability of Candida auris, a dangerous fungal pathogen. These cell viability assays are crucial for testing antifungal drugs and disinfectants effectively.

Keywords:
Candida aurisCell viabilityColony-forming unitsFluorescent stainingLive/dead qPCRResazurinXTT

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Area of Science:

  • Mycology
  • Antimicrobial Research
  • Infectious Diseases

Background:

  • Cell viability assays are essential for evaluating antifungal treatments and disinfection efficacy.
  • The nosocomial fungal pathogen Candida auris requires robust methods for therapeutic assessment.
  • Existing methods need standardization for reliable Candida auris research.

Purpose of the Study:

  • To provide detailed methodologies for assessing Candida auris cell viability in vitro.
  • To establish standardized protocols for antifungal drug screening against Candida auris.
  • To facilitate accurate efficacy testing of disinfection strategies targeting Candida auris.

Main Methods:

  • Metabolic assays, including XTT and resazurin assays, were employed.
  • Colony-forming unit counting was utilized for direct microbial enumeration.
  • Live/dead quantitative PCR and fluorescent staining coupled with microscopy were performed.

Main Results:

  • The study outlines the practical application of multiple viability assays for Candida auris.
  • Comparative data on the sensitivity and specificity of each method are implicitly discussed.
  • Successful implementation of these methods allows for quantitative assessment of fungal viability.

Conclusions:

  • Standardized cell viability assays are critical for advancing antifungal drug discovery.
  • Accurate assessment of Candida auris viability aids in developing effective control strategies.
  • These described methods support research into novel therapeutics and disinfectants for Candida auris infections.