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Bacterial conversion routes for lignin valorization.

He Liu1, Zhi-Hua Liu1, Ren-Kuan Zhang1

  • 1Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.

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|June 8, 2022
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Summary

Bacterial conversion offers a promising route for upgrading recalcitrant lignin into valuable products. This cascaded approach enhances lignin valorization feasibility and profitability by efficiently processing heterogeneous aromatic streams.

Keywords:
Bacterial conversionBiological funnelLignin valorizationLigninolytic enzymesSynthetic biologyTransporter

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Area of Science:

  • Biotechnology and Bioengineering
  • Renewable Energy and Materials Science
  • Synthetic Biology

Background:

  • Lignin, a vast renewable aromatic resource, presents significant potential for producing value-added chemicals.
  • Challenges in lignin valorization stem from its inherent recalcitrance and heterogeneity, hindering efficient industrial application.
  • Current methods struggle to effectively utilize the complex structure of lignin for targeted product synthesis.

Purpose of the Study:

  • To provide a systematic overview of bacterial lignin valorization using a cascaded conversion strategy.
  • To explore how bacterial systems can overcome the heterogeneity of lignin-derived aromatic streams.
  • To assess the techno-economic feasibility of bacterial conversion for lignin upgrading.

Main Methods:

  • Employing combinatorial depolymerization to generate a suitable aromatic stream for bacterial uptake.
  • Investigating bacterial active transport mechanisms for efficient assimilation of lignin-derived aromatics.
  • Analyzing intracellular metabolic pathways, including "biological funnel" and aromatic ring cleavage, for product biosynthesis.
  • Conducting techno-economic analysis to evaluate the viability of the proposed bacterial conversion route.

Main Results:

  • A combinatorial depolymerization strategy yields an aromatic stream amenable to bacterial conversion.
  • Bacterial active transport and intracellular pathways effectively process heterogeneous lignin-derived aromatics.
  • The bacterial cascaded route enables the biosynthesis of various value-added products from lignin.
  • Techno-economic analysis indicates improved feasibility for co-producing value-added products from lignin.

Conclusions:

  • Bacterial cascaded conversion routes offer a promising strategy for upgrading heterogeneous lignin.
  • This approach enhances the efficiency and profitability of lignin valorization.
  • Bacterial systems provide a versatile platform for converting lignin into diverse value-added products.