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Related Experiment Videos

Subcellular distribution of hypothalamic prolactin-like immunoreactivity.

N V Emanuele, L Metcalfe, T Lubrano

    Brain Research
    |March 31, 1987
    PubMed
    Summary

    Researchers found that prolactin-like immunoreactivity (PLI) in the rat hypothalamus is primarily located in neuronal fractions, suggesting a role as a brain neuromodulator.

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    Area of Science:

    • Neuroendocrinology
    • Cellular Biology
    • Neuroscience

    Background:

    • Immunohistochemical studies reported prolactin-like immunoreactivity (PLI) in the rat hypothalamus.
    • Previous work demonstrated the extraction of immunoreactive and bioactive prolactin-like material from the rat hypothalamus.

    Purpose of the Study:

    • To determine the subcellular distribution of prolactin-like material within the rat hypothalamus.
    • To investigate the cellular localization of hypothalamic PLI to understand its potential function.

    Main Methods:

    • Subcellular fractionation of neuronal tissue from rat hypothalamus.
    • Sensitive and specific radioimmunoassay for rat prolactin.
    • Discontinuous sucrose density gradient centrifugation for further fractionation of subcellular components.

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    Main Results:

    • 90% of hypothalamic PLI was found to be particulate-bound, with only 10% in the cytosolic fraction.
    • Approximately 80% of particulate-bound PLI was localized to the P2 fraction (myelin, synaptosomes, mitochondria).
    • Further fractionation of P2 revealed that 66% of PLI was associated with synaptosome-rich subfractions.

    Conclusions:

    • The majority of hypothalamic prolactin-like material is associated with synaptosomal fractions.
    • These findings support the hypothesis that hypothalamic PLI functions trans-synaptically as a neuromodulator in the brain.