Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

3.6K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
3.6K
Centrosome Duplication02:25

Centrosome Duplication

4.2K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.2K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.6K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.6K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.3K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.3K
Hematopoiesis01:21

Hematopoiesis

5.7K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
5.7K
Lineage Commitment01:21

Lineage Commitment

3.3K
Commitment is the  process whereby stem cells:
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ginger and Its Purified Major Components Inhibit Clinically Relevant Uptake and Efflux Transporters In Vitro.

Pharmaceutics·2026
Same author

CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2.

The EMBO journal·2024
Same author

Dynamic Interplay in Tumor Ecosystems: Communication between Hepatoma Cells and Fibroblasts.

International journal of molecular sciences·2023
Same author

The Inhibitor Preincubation Effect Is Universal to SLC Transporter Assays and Is Only Partially Eliminated in the Presence of Extracellular Protein.

Drug metabolism and disposition: the biological fate of chemicals·2023
Same author

A histone deacetylase 3 and mitochondrial complex I axis regulates toxic formaldehyde production.

Science advances·2023
Same author

Role of Hepatocyte Transporters in Drug-Induced Liver Injury (DILI)-In Vitro Testing.

Pharmaceutics·2023
Same journal

The TPR2 corepressor forms condensates with repressors to fine-tune growth and development in rice.

The EMBO journal·2026
Same journal

SenFlag gene signature identifies senescent cells in mouse and human tissues through a conserved core transcriptional program.

The EMBO journal·2026
Same journal

Scalable phosphotyrosine enrichment with SH2 superbinder enables deep profiling of EGF responses.

The EMBO journal·2026
Same journal

Essential nucleus-apical pole linkage maintains division fidelity during Plasmodium progeny formation.

The EMBO journal·2026
Same journal

From cell atlases to mechanisms: bridging scRNA-seq discovery with in vivo genetics.

The EMBO journal·2026
Same journal

Mitochondrial calcium regulates lipid metabolism by modulating tethering of mitochondria to lipid droplets.

The EMBO journal·2026
See all related articles

Related Experiment Video

Updated: Sep 20, 2025

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
06:40

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Published on: September 9, 2014

15.3K

Centrosome function is critical during terminal erythroid differentiation.

Péter Tátrai1, Fanni Gergely1,2

  • 1Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Cambridge, UK.

The EMBO Journal
|June 9, 2022
PubMed
Summary
This summary is machine-generated.

Centrosomes, crucial for cell division, are vital for normal red blood cell production. Their disruption, linked to CDK5RAP2 deficiency, causes macrocytic anemia by impairing cell division and leading to larger red blood cells.

Keywords:
bloodcentrosomeenucleationerythropoiesismitotic spindle

More Related Videos

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

16.1K
Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition
09:00

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Published on: June 16, 2019

9.7K

Related Experiment Videos

Last Updated: Sep 20, 2025

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
06:40

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Published on: September 9, 2014

15.3K
Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

16.1K
Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition
09:00

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Published on: June 16, 2019

9.7K

Area of Science:

  • Cell Biology
  • Hematology
  • Molecular Biology

Background:

  • Red blood cell production involves erythroblast differentiation into enucleated reticulocytes.
  • Microtubules are essential for enucleation, but the role of the centrosome remains unclear.

Purpose of the Study:

  • To investigate the role of the centrosome, specifically CDK5RAP2, in erythroid differentiation.
  • To determine if centrosome dysfunction contributes to macrocytic anemia.

Main Methods:

  • Studied CDK5RAP2-deficient mice exhibiting macrocytic anemia.
  • Analyzed fetal liver-derived erythroid progenitors and erythroblasts.
  • Utilized pharmacological centrosome depletion in erythroblasts.

Main Results:

  • CDK5RAP2-deficient erythroid progenitors produced fewer, larger reticulocytes, mimicking macrocytic anemia.
  • Loss of CDK5RAP2 or centrosomes caused aberrant spindle morphology in erythroblasts.
  • Tetraploidy was frequent in late-stage erythroblasts due to failed chromosome segregation.

Conclusions:

  • CDK5RAP2 and centrosomes are critical for proper spindle formation during erythroid differentiation.
  • Disrupted centrosome and spindle function may contribute to macrocytic anemias.
  • This finding has implications for understanding anemias related to nutritional deficiencies or chemotherapy.