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Related Experiment Video

Updated: Sep 20, 2025

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
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Structural and Functional Insights into GID/CTLH E3 Ligase Complexes.

Matthew E R Maitland1,2, Gilles A Lajoie2, Gary S Shaw2

  • 1Robarts Research Institute, Schulich School of Medicine & Dentistry, Western University, London, ON N6A 5B7, Canada.

International Journal of Molecular Sciences
|June 10, 2022
PubMed
Summary
This summary is machine-generated.

The C-terminal to LisH (CTLH) complex, a conserved E3 ligase, is crucial for protein degradation. This review synthesizes current knowledge on its structure and function, aiding therapeutic development.

Keywords:
CTLH complexE3 ligaseGID complexGID4RMND5ARanBP9/RanBPMWDR26muskelinubiquitination

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In Vitro Analysis of E3 Ubiquitin Ligase Function
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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Multi-subunit E3 ligases coordinate substrate receptors with a catalytic center for ubiquitin transfer.
  • Targeted protein degradation using small molecules has emerged as a therapeutic strategy for previously undruggable targets.
  • The C-terminal to LisH (CTLH) complex, also known as the glucose-induced degradation deficient (GID) complex, is a conserved multi-subunit E3 ligase involved in fundamental cellular processes.

Purpose of the Study:

  • To review existing literature on the CTLH complex across all organisms.
  • To contextualize findings on individual CTLH subunits within recent structural and functional discoveries.
  • To provide a comprehensive understanding of the CTLH complex's mechanistic basis.

Main Methods:

  • Literature review of studies on the CTLH/GID complex.
  • Synthesis of data on subunit interactions and functions.
  • Integration of structural and mechanistic findings.

Main Results:

  • The CTLH complex is highly conserved from yeast to humans.
  • It plays essential roles in homeostasis and development.
  • Recent breakthroughs have shed light on its structure and function.

Conclusions:

  • Understanding the CTLH complex's mechanism is crucial for its therapeutic potential.
  • This review consolidates current knowledge, highlighting gaps and future directions.
  • The CTLH complex represents a promising target for novel therapeutic interventions.