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Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Published on: February 27, 2015
Chemical Probes and Activity-Based Protein Profiling for Cancer Research
Mohammad Faysal Al Mazid1, Seung Bin Park2, Subba Rao Cheekatla2
1Division of Bio-Medical Science and Technology, Korea Institute of Science and Technology School, Korea University of Science and Technology, Seoul 02792, Korea.
Abstract:
Chemical probes can be used to understand the complex biological nature of diseases. Due to the diversity of cancer types and dynamic regulatory pathways involved in the disease, there is a need to identify signaling pathways and associated proteins or enzymes that are traceable or detectable in tests for cancer diagnosis and treatment. Currently, fluorogenic chemical probes are widely used to detect cancer-associated proteins and their binding partners. These probes are also applicable in photodynamic therapy to determine drug efficacy and monitor regulating factors. In this review, we discuss the synthesis of chemical probes for different cancer types from 2016 to the present time and their application in monitoring the activity of transferases, hydrolases, deacetylases, oxidoreductases, and immune cells. Moreover, we elaborate on their potential roles in photodynamic therapy.
Insights
This review highlights the development and application of chemical probes for cancer research. These probes aid in diagnosing cancer, monitoring treatment efficacy, and exploring new therapeutic strategies like photodynamic therapy.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Cancer's complexity necessitates identifying traceable signaling pathways and proteins for diagnosis and treatment.
- Fluorogenic chemical probes are crucial tools for detecting cancer-associated proteins and their interactions.
- These probes have applications in monitoring therapeutic responses and understanding disease mechanisms.
Purpose of the Study:
- To review the synthesis of chemical probes for various cancer types from 2016 to the present.
- To discuss the application of these probes in monitoring enzyme activities (transferases, hydrolases, deacetylases, oxidoreductases) and immune cells.
- To elaborate on the potential of chemical probes in photodynamic therapy for cancer treatment.
Main Methods:
- Literature review of chemical probe synthesis and applications in cancer research.
- Analysis of probe utility in detecting specific enzyme classes and immune cell functions.
- Exploration of probe integration into photodynamic therapy protocols.
Main Results:
- Significant advancements in chemical probe synthesis tailored for diverse cancer types.
- Demonstrated utility of probes in monitoring key enzymatic activities and immune responses relevant to cancer.
- Emerging applications of chemical probes in evaluating photodynamic therapy efficacy.
Conclusions:
- Chemical probes are versatile tools for advancing cancer diagnosis, treatment monitoring, and therapeutic development.
- Continued innovation in chemical probe design will enhance our understanding and management of cancer.
- Probes show promise for personalized medicine approaches in oncology and photodynamic therapy.
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