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Updated: Sep 20, 2025
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
22.1K
CRISPR-Cas technology a new era in genomic engineering
Ali Parsaeimehr1, Rosemary I Ebirim1, Gulnihal Ozbay1
1Department of Agriculture and Natural Resources, College of Agriculture, Science, and Technology, Delaware State University, Dover, DE 19901, United States of America.
Biotechnology Reports (Amsterdam, Netherlands)
|June 10, 2022
Summary
CRISPR-Cas gene editing technology offers precise genome modification for agriculture, medicine, and biofuels. Its ongoing evolution with regulators and anti-CRISPR molecules enhances control and safety, with ethical considerations discussed.
Area of Science:
- Molecular Biology
- Biotechnology
- Genetics
Background:
- CRISPR-Cas systems provide a versatile platform for precise genome modification.
- Applications span agricultural biotechnology, therapeutics, genetic disease research, crop improvement, and biofuel generation.
Purpose of the Study:
- To review current applications and advancements in CRISPR-Cas genome editing.
- To highlight the role of anti-CRISPR molecules in regulating CRISPR-Cas systems.
- To discuss ethical considerations surrounding CRISPR-Cas technology.
Main Methods:
- Literature review of CRISPR-Cas applications and research.
- Analysis of anti-CRISPR molecule mechanisms and regulatory functions.
- Discussion of ethical implications based on current research and potential impacts.
Main Results:
- CRISPR-Cas technology is rapidly advancing, offering increased precision and control.
- Anti-CRISPR molecules are emerging as key regulators for safe and effective gene editing.
- Ethical discussions are crucial for responsible implementation of CRISPR-Cas applications.
Conclusions:
- CRISPR-Cas systems are transformative tools with broad scientific and societal implications.
- Continued research into CRISPR-Cas evolution and regulation is vital.
- Ethical frameworks must guide the development and application of genome editing technologies.
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