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Updated: Sep 20, 2025

Introduction to Solid Supported Membrane Based Electrophysiology
Published on: May 11, 2013
Detection of cell membrane proteins using ion-sensitive field effect transistors combined with chemical signal
Miyuki Tabata1, Chattarika Khamhanglit1, Sayo Kotaki1
1Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan. miyahara.bsr@tmd.ac.jp.
Abstract:
The capture and detection of cells expressing a breast-cancer related membrane protein, namely a BT474 cell line expressing HER2, is demonstrated using ion-sensitive field effect transistors (ISFETs). BT474 cells were exposed to anti-HER2 antibodies and urease-conjugated secondary antibodies to induce chemical signal amplification by adding urea.
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