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Updated: Sep 20, 2025

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

DNA Nanoscaffolds for Multienzyme Systems Assembly.

Sung Won Oh^1,2, Zhicheng Wang^1,2, Jinglin Fu^3,4

¹Department of Chemistry, Rutgers University-Camden, Camden, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 10, 2022

Summary

This summary is machine-generated.

DNA nanoscaffolds precisely organize multienzyme systems for efficient cellular metabolic functions. This study details protocols for assembling these nanoscale enzyme complexes, improving substrate transport and function.

Keywords:

Bioconjugation and purification DNA nanostructure Gel electrophoresis Multienzyme assembly Nanoreactors Protein chromatography Self-assembly

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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

Area of Science:

Biochemistry
Nanotechnology
Synthetic Biology

Background:

Multienzyme reactions are crucial for cellular metabolism, often forming organized complexes called metabolons.
Metabolon assembly optimizes enzyme positioning and orientation for efficient substrate transport.
DNA nanotechnology offers precise nanoscale control for organizing biomolecular assemblies.

Purpose of the Study:

To present detailed protocols for assembling multienzyme systems using DNA nanoscaffolds.
To demonstrate control over the spatial interactions and arrangements of individual enzymatic components.
To enable the creation of artificial metabolons with enhanced functionality.

Main Methods:

Preparation and purification of DNA nanostructures.
Bioconjugation of DNA with proteins and cofactors.
Chromatography purification of DNA-conjugated biomolecules.
Characterization using gel electrophoresis and Atomic Force Microscopy (AFM).
Activity evaluation of the assembled multienzyme systems.

Main Results:

Successful assembly of multienzyme systems with controlled spatial arrangements.
Demonstrated feasibility of using DNA nanoscaffolds for precise enzyme organization.
Validated characterization and activity evaluation methods for DNA-protein assemblies.

Conclusions:

DNA nanoscaffolds provide a powerful platform for engineering functional multienzyme systems.
Protocols enable precise control over enzyme positioning, mimicking natural metabolons.
This approach has potential applications in synthetic biology and metabolic engineering.